calibration is also one of the system
suitability test,whether the system working satisfactory or
not physically so when you do the calibration for pH meter
with standard pH buffer solution (which is from NIST-it may
accurate solution for actual pH)and once if your instrument
has calibrated,you can cross verify your insrument against
those pH buffer solution (NIST) and the acceptable range is
+/- 0.02 then you can conclude for ?
once if temperature raises the pH will decrease and its not
all cases
#Base line is nothing but the detectors response to the
mobile phase.(gc,hplc)
#Base line should be stable to start a run.
#Un stabilized base line is called as base line noise.
#Base line noise is of many types..
1)Cyclic base line
2)Synchorinise noise
3)Asynchorinise noise
4)Base line drift
5)Spikes
6)Negative peaks
7)No peaks
#Base line noise is attributed due to mobile phase,sample,
system problems.leaks in the system,temperature variations
in lab etc..
The chemical substances that pair each other, to form
complexes. These can use for stabilization of one of the
molecule that is more active or to colorifying etc.
The H is the height of the main peak and the h is the lowest
height of a peak in the range of X5 the main peak (around
the amin peak)
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