Bio Chemistry Interview Questions and Answers

Bio Chemistry Questions and Answers:

1 :: How does the formation of the enzyme-substrate complex explain the reduction of the activation energy of chemical reactions?

The enzyme possibly works as a test tube within which reagents meet to form products. With the facilitation of the meeting provided by enzymes it is easier for collisions between reagents to occur and thus the activation energy of the chemical reaction is reduced. This is one of the explanatory hypotheses.
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2 :: What are the main theoretical models that try to explain the formation of the enzyme-substrate complex?

There are two main models that explain the formation of the enzyme-substrate complex the lock and key model and the induced fit model.

In the lock and key model, the enzyme has a region with specific spatial conformation for the binding of the substrate. In the induced fit model, the binding of the substrate induces a change in the spatial configuration of the enzyme for the substrate to fit.

Enzyme Activity: lock and key model induced fit model
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3 :: What is meant by substrates of enzymatic reactions?

Substrates are reagent molecules upon which enzymes act.

The enzyme has spatial binding sites for the attachment of its substrate. These sites are called activation centers of the enzyme. Substrates bind to theses centers forming the enzyme-substrate complex.

Enzyme Activity: enzyme-substrate complex
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4 :: What are enzymes? What is the importance of enzymes for the living beings?

Enzymes are proteins that are catalysts of chemical reactions. From Chemistry, it is known that catalysts are non-consumable substances that reduce the activation energy necessary for a chemical reaction to occur.

Enzymes are highly specific to the reactions they catalyze. They are of vital importance for life because most part of chemical reaction of the cells and tissues are catalyzed by enzymes. Without enzymatic action, those reactions would not occur or would not happen in the required speed for the biological processes in which they participate.
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5 :: What amount of catalyst is consumed in the reaction it catalyzes?

Catalysts are not consumed in the reactions they catalyze.
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