Bio Technology Interview Questions and Answers

Bio Technology Questions and Answers:

1 :: How does the DBM paper entrap the rna probes?

rna is having _ve charge & DBM paper having positive chrg
hence due to thease opposite charges DBM paper binds to RNA
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2 :: What is the nature of DBM paper in northern blotting?

positively charged i.e,cationic nature
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3 :: Why do you use DBM instead of NCF in the northern blotting and what are the pre-treatments required for this paper to be used?

diazobenzyloxymethyl paper that binds all single-stranded DNA, RNA, and proteins by means of covalent linkages to the diazonium group; used in situations where nitrocellulose blotting is not technically feasible
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4 :: What is the main objective of northern blotting?

it is used to determine the protein from a mixture of compounds
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5 :: DOES EUKARYOTES HAVE RESTRICTION MODIFICATION SYSTEM? IF YES, THAN WHY ARE WE NOT USING FOR THE TREATMENT OF HIV?

eukaryotes does not contain restriction modificayion system
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