Genetics Interview Question:

What is the method used to identify your transformed cells from the plasmid vector?

Submitted by: Administrator

I) If transformant is thought to have antibiotic resistant
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
Submitted by: Administrator