Biological Sciences Interview Question:

Why does separation not occurs in stacking gel in gel electrophoresis and why does separation done in separating gel?

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Stacking gel has a much larger pore size,and is merely used for stacking (arranging one over other) the samples so that they can be resolved in the separating gel easily. Since the separating gel has an optimal pore size so the samples separate by molecular sieving effect of the resolving gel which is absent in case of Stacking gel.
Submitted by: Administrator