Biomedical Engineer Interview Question:

What is superiority of TLC over paper chromatography?

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TLC is superior over paper chromatography because of inorganic nature of adsorbent concentrated sulfuric acid spray. The spray is then followed by heating. It may be used to develop on the chromatogram by charring. Also, amino acid mixtures require 18 hours for separation on paper. It requires 3 hrs using cellulose TLC. The advantages of TLC lie in adsorbents which don't allow separation on paper. In TLC we have much wider choice of adsorbents depending upon needs and sample.
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