Operational Immunology Interview Questions & Answers:

1. Expand cell line HL-60.

Human myeloid leukemia cell line

2. How B cell hybridomass are formed?

They are formed by the fusion of antigen primed B cells with cancerous plasma cells.

3. Name the assay method for insulin in serum.

ELISA or RIA

4. Name the assay method for horsemeat combination of hamburger.

Agglutination

5. Name the assay method for compliment component C3 on glomerullar basement membrane.

Immunofluorescence

6. Name the assay method for IgG in serum.

The method is ELISA.

7. What does the following sentence means? T cell is said to be class I restricted.

It means that they can recognize the antigen, which is, associated with class I MHC molecules.

8. Describe major events in the inflammatory response.

The following are the major events in the inflammatory response:
The diameter of the capillaries increases in the affected region and their permeability, which facilitates influx of white blood cells.

9. Name some features of a secondary immune response that distinguish it from primary immune response

Secondary immune response requires an amplified population of memory cells. Response is more rapid compared to primary immune response.

10. Give an example for alloantigen

Blood group antigens are alloantigens.