Bacteriologist Interview Question:

Tell me what Are The Conditions When Gram Positive Bacteria Can Appear Gram Negative?

Submitted by: Muhammad

☛ When over-decolourized by either prolonged exposure to decolourizer or using acetone alone.
☛ When cell wall gets damaged by exposure to lysozyme or cell wall acting antibiotics such as Penicillin.
☛ Old cultures, where cell wall is weakened or action of autolytic enzymes
☛ Those bacteria that are phagocytosed. where cell wall is acted upon by lysosomal contents
Submitted by: Muhammad