Bacteriologist Interview Question:

Tell us what Are The Precautions To Be Taken While Preparing Or Observing Smears For Afb?

Submitted by: Muhammad

☛ A new slide must be used for every specimen. because scratch marks may give false positive.
☛ A uniform smear from thick portion of the sputum must be made.
☛ Staining jars should not be used to staining smear as there is risk to cross contamination
☛ Fresh blotting paper must be used for each smear for drying the slide to prevent transfer from one slide to another.
Submitted by: Muhammad