What is the yous of HPLC, how we use the columns, what is the important in the pharma field?

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HPLC is high performance liquid chromatography,it is used
for analytical purpose for identification of impurity in
drugs during its synthesis. during preparation phase it
helps in understanding the solvent to be chosen and RT of
the component. it can also separate different component of
drug based on their Retention time. RT varies with column
used and the mobile phase used.
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