Genetics Interview Questions and Answers

Genetics Questions and Answers:

1 :: The three stop codons UAG, UAA, UGA do not code for any proteins. then why does the start codon AUG code for a protein, i.e, methionine?

AUG codon codes for an amino acid ;Methionine, because a tRNA is available with the anti-codon complementary to the AUG codon. This tRNA brings in the Methionine molecule when charged to the site of protein synthesis ie Ribosomes in the cytosol.
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2 :: Who was the father of genetic engineering?

Paul Berg
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3 :: Is male child having more genital sequence as of father or mother?

Male child is having the genital sequence equally as the
mother and father because in male child the x-chromosome
comes from mother and the y-chromosome comes from father
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4 :: What is the method used to identify your transformed cells from the plasmid vector?

I) If transformant is thought to have antibiotic resistant
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
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5 :: Explain steps involved in transcription?

there are 3 basic steps initiation, elongation and termination
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