1. What is the difference between plasma membrane and cell wall?

Plasma membrane and cell wall is not the same thing. Plasma membrane, also called cell membrane, is the outer membrane common to all living cells and it is made of a phospholipid bilayer, embedded proteins and some appended carbohydrates.

Because cell membranes are fragile, in some types of cells there are even outer structures that support and protect the membrane, like the cellulose wall of plant cells and the chitin wall of some fungi cells. Most bacteria also present an outer cell wall made of peptidoglycans and other organic substances.

Cell Structure Review - Image Diversity: cell wall

2. What are the chemical substances that compose the plasma membrane?

The main constituents of the plasma membrane are phospholipids, proteins and carbohydrates. The phospholipds, amphipathic molecules, are regularly organized in the membrane according to their polarity: two layers of phospholipids form the lipid bilayer with the polar part of the phospholipids pointing to the exterior of the layer and the non polar phospholipid chains in the interior. Proteins can be found embedded in the lipid bilayer and there are also some carbohydrates bound to proteins and to phospholipids in the outer face of the membrane.

3. Do bacteria cells have nucleus?

In bacteria the genetic material is dispersed in the cytosol and there is no internal membrane that delimits a nucleus.

4. Is there any bacteria made of more than one cell?

There are no pluricellular bacteria. All bacteria are unicellular prokaryotic.

5. What is the plasma membrane of the cell? What are its main functions?

The plasma membrane is the outer membrane of the cell it delimits the cell itself and a cell interior with specific conditions for the cellular function. Since it is selectively permeable the plasma membrane has an important role for the passage of substances inwards or outwards.

Cell Structure Review - Image Diversity: cell membrane

6. What are the two big groups into which cells are classified?

Cells can be classified as eukaryotic or prokaryotic.

Prokaryotic cell is that without a delimited nucleus. Eukaryotic cells are those with nucleus delimited by membrane.

Cell Structure Review - Image Diversity: eukaryotic cell prokaryotic cell

7. In 1665 Robert Hooke, an English scientist, published his book Micrographia, in which he described that pieces of cork viewed under the microscope present small cavities similar to pores and filled with air. Based on later knowledge of what were the walls of those cavities constituted? What is the historical importance of that observation?

The walls of the cavities observed by Hooke were the walls of the plant cells that form the tissue. The observation leaded to the the discovery of the cells, a fact only possible after the invention of the microscope. In that work, Hooke established the term “cell”, now widely used in Biology, to designate those cavities seen under the microscope.

Cell Structure Review - Image Diversity: Hooke's cell

8. Are there living beings without cell?

The virus is considered the only alive beings that do not have cells. Virus are constituted by genetic material (DNA or RNA) enwrapped by a protein capsule. They do not have membrane and cell organelles neither self-metabolism.

9. What is the cell theory?

Cell theory is a theory that asserts that the cell is the constituent unit of the living beings.

Before the discovery of the cell, it was not recognized that the living beings were made of building blocks like cells.

The cell theory is one of the basic theories of Biology.

10. What are the main respective constituents of cell walls in bacteria, protists, fungi and plants?

In bacteria cell wall is made of peptidoglycans; among protists algae have cell wall made of cellulose; in fungi, the cell wall is made of chitin (the same substance that makes the exoskeleton of arthropods); in plants, the cell wall is made of cellulose too.

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11. Do membranes form only the outer wrapping of cells?

Lipid membranes do not form only the outer cover of cells. Cell organelles, such as the Golgi complex, mitochondria, chloroplasts, lysosomes, the endoplasmic reticula and the nucleus, are delimited by membranes too.

Cell Structure Review - Image Diversity: cell nucleus

12. Which type of cell came first in evolution the eukaryotic cell or the prokaryotic cell?

This is an interesting problem of biological evolution. The most accepted hypothesis asserts that the more simple cell, the prokaryotic cell, appeared early in evolution than the more complex eukaryotic cell. The endosymbiotic hypothesis, for example, affirms that aerobic eukaryotic cells appeared from the mutualist ecological interaction between aerobic prokaryotes and primitive anaerobic eukaryotes.

13. Concerning the presence of nucleus what is the difference between animal and bacterial cells?

Animal cells (cells of living beings of the kingdom Animalia) have an interior membrane that delimits a cell nucleus and thus they are eukaryotic cells; in these cells the genetic material is located within the nucleus. Bacterial cells (cells of living beings of the kingdom Monera) do not have organized cellular nucleus and so they are prokaryotic cells and their genetic material is found dispersed in the cytosol.

14. What are the three main parts of a eukaryotic cell?

The eukaryotic cell can be divided into two main portions: the cell membrane that separates the intracellular space from the outer space phisically delimiting the cell; the cytoplasm, the interior portion filled with cytosol (the aqueous fluid inside the cell); and the nucleus, the membrane-delimited internal region that contains the genetic material.

15. What are the main structures within the cell nucleus?

Within the cell nucleus the main structures are: the nucleolus, an optically dense region, spherical shaped, where there are concentrated ribosomal RNA (rRNA) associated to proteins (there may be more than one nucleolus in a nucleus); the chromatin, made of DNA molecules dispersed in the nuclear matrix during the cell interphase; the karyotecha, or nuclear membrane, the membrane that delimits the nucleus.

16. What are the substances that constitute the chromatin? What is the difference between chromatin and chromosome?

The chromatin, dispersed in the nucleus, is a set of filamentous DNA molecules associated to nuclear proteins called histones. Each DNA filament is a double helix of DNA and thus a chromosome.

17. How is the fluid that fills the nucleus called?

The aqueous fluid that fills the nuclear region is called karyolymph, or nucleoplasm. In the fluid there are proteins, enzymes and other important substances for the nuclear metabolism.

18. Of what substances is the nucleolus made? Is there a membrane around the nucleolus?

Nucleolus is a region within the nucleus made of ribosomal RNA (rRNA) and proteins. It is not delimited by membrane.

19. What is the name of the membrane that delimits the nucleus? To which component of the cell structure that membrane is contiguous?

The nuclear membrane is also called karyotheca. The nuclear membrane is continuous to the endoplasmic reticulum membrane.

20. What are the main cytoplasmic structures present in animal cells?

The main cytoplasmic structures of the cell are the centrioles, the cytoskeleton, lysosomes, mitochondria, peroxisomes, the Golgi apparatus, the endoplasmic reticula and ribosomes.

Cell Structure Review - Image Diversity: cell organelles

21. What are cytoplasmic inclusions?

Cytoplasmic inclusions are cytoplasmic molecular aggregates, such as pigments, organic polymers and crystals. They are not considered cell organelles.

Fat drops and glycogen granules are examples of cytoplasmic inclusions.

Cell Structure Review - Image Diversity: cytoplasmic inclusions

22. Where in the cell can ribosomes be found? What is the main biological function of ribosomes?

Ribosomes can be found free in the cytoplasm, adhered to the outer side of the nuclear membrane or associated to the endoplasmic reticulum membrane defining the rough endoplasmic reticulum. Ribosomes are the structures where protein synthesis takes place.

Cell Structure Review - Image Diversity: ribosomes

23. What is the difference between smooth and rough endoplasmic reticulum?

The endoplasmic reticulum is a delicate membranous structure contiguous to the nuclear membrane and present in the cytoplasm. It forms an extense net of channels throughout the cell and it is divided in rough and smooth types.

The rough endoplasmic reticulum has great amount of ribosomes adhered to the external side of its membrane. The smooth endoplasmic reticulum does not have ribosomes attached to its membrane.

The main functions of the rough endoplasmic reticulum are synthesis and storage of proteins made in the ribosomes. The smooth endoplasmic reticulum plays a role in the lipid synthesis and, in muscle cells it is importante in the conduction of the contraction stimulus.

Cell Structure Review - Image Diversity: endoplasmic reticulum

24. A netlike membranous complex of superposed flat saccules with vesicles detaching from the extremities seen in electronic microscopy. What is the observed structure? What is its biological function?

What is being observed is the Golgi complex, or Golgi apparatus. This cytoplasmic organelle is associated with chemical processing and modification of proteins made by the cell and with storage and branding of these proteins for posterior use or secretion. Vesicles seen in the electronic microscope contain material already processed, ready to be exported (secreted) by the cell. The vesicles detach from the Golgi apparatus, travel across the cytoplasm and fuse with the plasma membrane then secreting their substances to the exterior.

Cell Structure Review - Image Diversity: Golgi apparatus

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25. On which organelle of the cell structure does intracellular digestion depends? What is the chemical content of those organelles?

Intracellular digestion occurs by the action of lysosomes. Lysosomes have digestive enzymes (hydrolases) that are made in the rough endoplasmic reticulum and stored in the Golgi apparatus. Lysosomes are hydrolase-containing vesicles that detach from the Golgi apparatus.