3. Explain How to estimate protein?

when Ninhydrin (triketohydrindene hydrate)reacting with free
amines,a deep blue or purple color known as Ruhemann's
purple is evolved.Ninhydrin test is most commonly used to
estimate proteins.

4. How would u measure carbohydrate?

1: by molish test
2:iodine reaction give blue colour with starch

5. Explain what is difference between southern western and northern blotting

southern blotting is for DNA
WESTERN FOR PROTEINS
ANE NORTHERN FOR RNA