Top Electron Microscopist Interview Questions & Answers:

1. Which is most correct in this question?

No Answer is Posted For this Question

2. The nuclear pores are passageways between the nuclear matrix and the cytoplasm which
A) allow DNA to reach the cytoplasm to be translated to RNA.
B) allow ribosomes to enter the nuclear matrix.
C) allow proteins synthesized in the cytoplasm to enter the nucleus.
D) structurally reinforce the nuclear membrane

C) allow proteins synthesized in the cytoplasm to enter the nucleus.

3. All the cells chromosomes are contained in the cell nucleus for:
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

B) eucaryotes only.

4. Exoenzymes are contained in the periplasmic space in:
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

A) procaryotes only.

5. Ribosomes are required for synthesis of proteins in:
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

C) both procaryotes and eucaryotes

6. Membrane bound organelles such as mitochondria and Golgi are found in
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

B) eucaryotes only.

7. When flagella exist, their movement is powered by ATP in
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

B) eucaryotes only.

8. Cell walls, when they exist, usually contain peptidoglycan in:
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

A) procaryotes only.

9. DNA is the primary molecule for information storage in
A) procaryotes only.
B) eucaryotes only.
C) both procaryotes and eucaryotes

C) both procaryotes and eucaryotes

10. The nucleus of the cell is where
A) energy for cell division is generated.
B) ribosomes translate mRNA into proteins.
C) chromatin is kept within a membrane delimited body.
D) endocytic vacuoles fuse with lysosomes

C) chromatin is kept within a membrane delimited body.

11. The F1 particles of mitochondria are:
A) a type of ribosome that synthesizes mitochondrial proteins.
B) a structural component of the mitochondrial inner membrane.
C) an enzyme complex that synthesizes ATP during respiration.
D) a storage complex for calcium phosphate in the mitochondria

C) an enzyme complex that synthesizes ATP during respiration.

12. Plastids are
A) protrusions of plasma membrane that bud off of plant cells.
B) cytoplasmic organelles that often contain photosynthetic pigments.
C) pieces of circular, double stranded DNA that can carry drug resistance.
D) areas of the nucleus where rRNA is transcribed

B) cytoplasmic organelles that often contain photosynthetic pigments.

13. Mitochondria are similar to bacteria in several ways. Which of the following is NOT a similarity?
A) Both have peptidyl glycan in their cell wall.
B) Both have circular, double stranded DNA.
C) Both are about the same size.
D) Both have 70S ribosomes

A) Both have peptidyl glycan in their cell wall.

14. Lysosomes of the cell are where
A) lysozyme is synthesized.
B) secretory proteins are transported to the surface.
C) endocytosed material is degraded.
D) glycogen is stored as an energy source

C) endocytosed material is degraded.

15. Colchicine treatment of cells to disrupt microtubules can result in
A) loss of cell shape
B) loss of directional movement.
C) loss of nutrient transport.
D) all of the above

D) all of the above

16. Which of the following is NOT a mechanism for a cell to degrade its own cytosolic proteins?
A) Autophagic vacuoles
B) Phagocytosis and secondary lysosome fusion.
C) 26S Proteosome degradation.
D) All the above are correct mechanisms

B) Phagocytosis and secondary lysosome fusion.

17. When Listeria monocytogenes invades a eucaryotic cell, it secretes the ActA protein that:
A) polymerizes actin filaments, causing the bacterium to move through the cytoplasm.
B) depolymerizes microfilaments, allowing the bacterium to move more easily in the cytoplasm.
C) degrades the cytoskeleton, allowing the bacterium to travel through the plasma membrane to adjacent cells.
D) attaches to microtubules, allowing the bacterium to move along the fibers

A) polymerizes actin filaments, causing the bacterium to move through the cytoplasm.

18. The cytoplasmic matrix is best described as:
A) A dilute aqueous solution that fills most of the cell.
B) A solution of inorganic salts intended to balance osmotic pressure in the cell.
C) A semicrystalline mixture mostly of proteins and bound water molecules.
D) Material in transit between membrane bound organelles

C) A semicrystalline mixture mostly of proteins and bound water molecules.

19. Which of the following statements about Endoplasmic reticulum (ER) is not true?
A) ER can transport proteins and lipids to the plasma membrane or for secretion.
B) ER is the main site for cell membrane biosynthesis.
C) ER is contiguous with the nuclear membrane.
D) ER is the main site for synthesis of cytoplasmic proteins

D) ER is the main site for synthesis of cytoplasmic proteins

20. The mitochondria is the site of:
A) protein synthesis
B) lipid synthesis
C) energy production

C) energy production

21. The diploid state of a microorganism refers to the times when cells contain _______ copy (ies) of each chromosome and occurs after mitotic cell division.
A) one
B) two
C) three

B) two

22. Bacterial flagella are too small to be seen by light microscopy without staining the specimen.
A) True
B) False

A) True

23. Acid fast stains interact with the waxy structures of the cell wall of Mycobacterium species.
A) True
B) False

A) True

24. Negative stains color the structures of interest a deep black.
A) True
B) False

B) False

25. Transmission electron microscopy has a practical magnification of greater than 100,000x.
A) True
B) False

A) True

26. Chromogenic stains which consist of a single dye rather than several dyes in combination are known as basic stains .
A) True
B) False

B) False

27. Fluorescence microscopy may be used with specially stained antibodies to detect specific microorganisms.
A) True
B) False

A) True

28. Fluorescence microscopy must be done on fixed and stained microorganisms.
A) True
B) False

B) False

29. Darkfield microscopy is best for visualizing live, motile microorganisms.
A) True
B) False

A) True

30. Even on the best light microscopes, increasing total magnification beyond 1500 cannot result in better resolution of the image.
A) True
B) False

A) True

31. Objective lenses with higher magnification generally have a shorter working distance.
A) True
B) False

A) True

32. The fluorescence microscope has become an essential tool in medical microbiology to identify bacterial pathogens.
A) True
B) False

A) True

33. Microscope resolution increases as the wavelength of radiation used to illuminate the specimen decreases.
A) True
B) False

A) True

34. In a compound microscope, the primary image is formed by an objective lens and enlarged by the optical lens to reveal a virtual image.
A) True
B) False

A) True

35. Differential Interference Contrast microscopy
A) compares two identical specimens on the same microscope.
B) illuminates the specimen with light of two different colors.
C) illuminates the specimen with light of two different phases.
D) illuminates the specimen with both reflected and transmitted light

C) illuminates the specimen with light of two different phases.