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Electron Microscopist Interview Question:

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Which of the following statements about Transmission Electron Microscopy is not true.
A) The specimen must be stained with osmium or other heavy metal.
B) The specimens are placed in a high vacuum for viewing.
C) The specimens must be sliced very thin, 20-100 nm in thickness.
D) The beam is focused by electromagnetic lenses

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A) The specimen must be stained with osmium or other heavy metal.
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Which of the following statements is most correct about Atomic Force Microscopy (AFM)?
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B) AFM can visualize unfixed specimens in water or buffer.
C) AFM moves a very sharp tip over the surface of the specimen to "feel" its shape.
D) All the statements above are true

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