Immunology Interview Question:

What is double immuno diffusion method?

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In this method, both antigens and antibodies diffuse radically from wells towards each other by establishing a concentration gradient. As equivalence is reached, a visible line of precipitation is observed.

The patterns of precipitin lines that are formed when two different antigens are placed in adjacent wells indicate whether they share any common epitope or not.

Identity occurs when two antigens share identical epitopes; hence, the line of precipitation formed by them will fuse to give single curve line of identity.

Non-identity occurs when two antigens are unrelated. The antiserum form independent precipitin lines that cross each other.

Partial identity occurs when two antigens share common epitope. The antiserum forms line of identity with the common epitope and a curved spur with the unique epitope.
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